| Place | Performance | Athlete | Team |
| 1 | 0:43.03 | Q: RELAY TEAM - | SASOLBURG HOËRSKOOL |
| 2 | 0:43.75 | Q: RELAY TEAM - | HTS SASOLBURG |
| 3 | 0:45.63 | Q: RELAY TEAM - | FICHARDPARK HS |
| 4 | 0:45.88 | Q: RELAY TEAM - | SALOMON SENEKAL HS |
| 5 | 0:46.98 | -: RELAY TEAM - | VIRGINIA HS |
| 6 | 0:47.41 | -: RELAY TEAM - | AFRIKAANS HS SASOLBURG |